rabbit polyclonal antibody against human agr2  (Cell Signaling Technology Inc)

Journal: International Journal of Clinical and Experimental Pathology

Article Title: Associations of MACC1, AGR2, and KAI1 expression with the metastasis and prognosis in head and neck squamous cell carcinoma

doi:

Figure Lengend Snippet: Expression of the patients in head and neck squamous cell carcinoma (×400 magnification). A. Negative MACC1 expression in the control normal cells. B. Positive MACC1 expression in the cytoplasm of cancer cells. C. Negative AGR2 expression in the control normal cells. D. Positive AGR2 expression in the nuclei of cancer cells. E. Positive KAI1 expression in the membrane and cytoplasm of control normal cells. F. Negative KAI1 expression in cancer cells.

Article Snippet: Subsequently placed in citrate buffer (pH 6.0) at 95°C to repair antigen for 30 min. After several washes with PBS, all slices were blocked by goat serum for 30 min, subsequently incubated with rabbit polyclonal antibody against human MACC1 (Santa Cruz Biotechnology, CA, USA) or rabbit polyclonal antibody against human AGR2 (Cell Signaling Technology, Inc., Danvers, MA, USA) or mouse monoclonal antibody against human KAI1 (Abcam Cambridge, MA, USA) at 37°C for 1 h. Finally, all slices were counterstained with hematoxylin, dehydrated, air-dried, and mounted.

Techniques: Expressing, Control, Membrane

Journal: International Journal of Clinical and Experimental Pathology

Article Title: Associations of MACC1, AGR2, and KAI1 expression with the metastasis and prognosis in head and neck squamous cell carcinoma

doi:

Figure Lengend Snippet: The associations between expression of MACC1, AGR2, and KAI1 and clinicopathological characteristics of head and neck squamous cell carcinoma (HNSCC)

Article Snippet: Subsequently placed in citrate buffer (pH 6.0) at 95°C to repair antigen for 30 min. After several washes with PBS, all slices were blocked by goat serum for 30 min, subsequently incubated with rabbit polyclonal antibody against human MACC1 (Santa Cruz Biotechnology, CA, USA) or rabbit polyclonal antibody against human AGR2 (Cell Signaling Technology, Inc., Danvers, MA, USA) or mouse monoclonal antibody against human KAI1 (Abcam Cambridge, MA, USA) at 37°C for 1 h. Finally, all slices were counterstained with hematoxylin, dehydrated, air-dried, and mounted.

Techniques: Expressing

Journal: International Journal of Clinical and Experimental Pathology

Article Title: Associations of MACC1, AGR2, and KAI1 expression with the metastasis and prognosis in head and neck squamous cell carcinoma

doi:

Figure Lengend Snippet: Association between expression of MACC1, AGR2, and KAI1 in HNSCC

Article Snippet: Subsequently placed in citrate buffer (pH 6.0) at 95°C to repair antigen for 30 min. After several washes with PBS, all slices were blocked by goat serum for 30 min, subsequently incubated with rabbit polyclonal antibody against human MACC1 (Santa Cruz Biotechnology, CA, USA) or rabbit polyclonal antibody against human AGR2 (Cell Signaling Technology, Inc., Danvers, MA, USA) or mouse monoclonal antibody against human KAI1 (Abcam Cambridge, MA, USA) at 37°C for 1 h. Finally, all slices were counterstained with hematoxylin, dehydrated, air-dried, and mounted.

Techniques: Expressing

Journal: International Journal of Clinical and Experimental Pathology

Article Title: Associations of MACC1, AGR2, and KAI1 expression with the metastasis and prognosis in head and neck squamous cell carcinoma

doi:

Figure Lengend Snippet: Kaplan-Meier analysis of the overall survival (OS) time of patients with head and neck squamous cell carcinoma. A. OS time of all patients in relation to MACC1 expression (log-rank =30.451, P<0.001). B. OS time of all patients in relation to AGR2 expression (log-rank =17.846, P<0.001). C. OS time of all patients in relation to KAI1 expression (log-rank =52.719, P<0.001). The green line represents positive expression of MACC1, AGR2, and KAI1 and the blue line represents negative expression of MACC1, AGR2, and KAI1.

Article Snippet: Subsequently placed in citrate buffer (pH 6.0) at 95°C to repair antigen for 30 min. After several washes with PBS, all slices were blocked by goat serum for 30 min, subsequently incubated with rabbit polyclonal antibody against human MACC1 (Santa Cruz Biotechnology, CA, USA) or rabbit polyclonal antibody against human AGR2 (Cell Signaling Technology, Inc., Danvers, MA, USA) or mouse monoclonal antibody against human KAI1 (Abcam Cambridge, MA, USA) at 37°C for 1 h. Finally, all slices were counterstained with hematoxylin, dehydrated, air-dried, and mounted.

Techniques: Expressing

Journal: International Journal of Clinical and Experimental Pathology

Article Title: Associations of MACC1, AGR2, and KAI1 expression with the metastasis and prognosis in head and neck squamous cell carcinoma

doi:

Figure Lengend Snippet: Results of multivariate analyses of overall survival (OS) time

Article Snippet: Subsequently placed in citrate buffer (pH 6.0) at 95°C to repair antigen for 30 min. After several washes with PBS, all slices were blocked by goat serum for 30 min, subsequently incubated with rabbit polyclonal antibody against human MACC1 (Santa Cruz Biotechnology, CA, USA) or rabbit polyclonal antibody against human AGR2 (Cell Signaling Technology, Inc., Danvers, MA, USA) or mouse monoclonal antibody against human KAI1 (Abcam Cambridge, MA, USA) at 37°C for 1 h. Finally, all slices were counterstained with hematoxylin, dehydrated, air-dried, and mounted.

Techniques: